|Step 1. The sample material (including the test antigen) is bound to a plastic plate.
Step 2. Monoclonal antibody (mAb) is then applied and left to bind to its specific antigen.
Step 3. The plate is then washed with buffer solution.
Step 4. A second antibody which will recognise and bind to the mAb-antigen complexes is then applied. Attached to this second antibody is an enzyme which can convert its substrate to a coloured product.
Step 5. The enzyme's substrate is added to the ELISA plates which allows both qualitative and quantitative assays of the test antigen to be obtained.